C2549-29A-BIOTIN
CD301 (CD301 Antigen, C-type Lectin Domain Family 10 Member A, CLEC10A, CLECSF13, C-type Lectin Superfamily Member 14, CLECSF14, HML2, Macrophage Lectin 2) (Biotin)
Size
£1,081.00
- SKU:
- C2549-29A-BIOTIN
- Purity:
- Purified by Protein G affinity chromatography from tissue culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rat
- Immunogen:
- Balb/c macrophage precursor cell hybrids.
- Clone:
- 10B1349 (ER-MP23)
- Format:
- Supplied as a liquid in PBS, pH 7.2. Labeled with Biotin.
- Extra Details:
- CD301 is a 38kD cell surface protein that is otherwise known as macrophage galactose N-acetylgalactosamine lectin (MGL) or dendritic cell asialoglycoprotein (DC-ASGPR). In mice, CD301 is predominantly expressed on mature macrophages found associated with a wide range of connective tissues including macrophages in the dermis and the pancreas. Expression of CD301 is induced by alternative (i.e. IL-4/IL-13 mediated) activation of macrophages and dendritic cells, but not all CD301 positive cells are necessarily IL-4/IL-13 stimulated. Applications: Suitable for use in Flow Cytometry, Immunofluorescence and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry: Frozen sections Immunohistochemistry (Paraffin): Heat mediated antigen retrieval is required prior to staining paraffin-embedded sections using citrae buffer, pH 6.0. Optimal dilutions to be determined by the researcher. Hybridoma: Rat Y3-Ag1.2.3 myeloma cells fused with cells from immunized rats. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice