C2549-21C1
CD273 (B7DC, Programmed Cell Death 1 Ligand 2, PD-1 Ligand 2, Programmed Death Ligand 2, PDL2) (Azide Free/Low Endotoxin)
Size
£510.00
- SKU:
- C2549-21C1
- Application:
- Flow Cytometry
- Purity:
- Purified by affinity chromatography. Endotoxin: ≤0.01EU/ug (LAL)
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rat
- Immunogen:
- Mouse B7-DC transfected cell line
- Clone:
- 10B1184 (TY25)
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservative added.
- Extra Details:
- B7-DC is also called programmed death ligand 2 (PDL2). It has recently been clustered as CD273. This ligand is a 42 kD member of the immunoglobulin receptor superfamily expressed on a subset of dendritic cells, liver and a small subset of macrophages as well as a few transformed cell lines. CD273 has been reported to be stimulatory on dendritic cells when cross-linked and to inhibit T cell activation upon engaging the PD-1 receptor. CD273 has also been reported to bind to an alternative receptor and to mediate T cell activation through such non-PD1 mediated interactions. C2549-21C1 has been reported to be useful for blocking PD-1 mediated interactions. Applications: Suitable for use in Flow Cytometry and Blocking. Though not tested, Immunohistochemistry, Immunoprecipitation and Western Blot have been reported in literature. Other applications not tested. Recommended Dilutions: Flow Cytometry: ≤ 0.25ug labels 10e6 cells in 100ul Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice