C2549-10A1

CD257 (B Lymphocyte Stimulator, BLyS, B Cell-activating Factor, BAFF, Dendritic Cell-derived TNF-like Molecule, DTL, THANK, TNF- and APOL-related Leukocyte Expressed Ligand 1, TALL1, TALL-1, TNFSF20, Tumor Necrosis Factor Ligand Superfamily Member 13B, TNFSF13B, UNQ401/PRO738, ZTNF4)

Size

£557.00

SKU:: C2549-10A1
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant corresponding to soluble human CD257
Clone:: 7H149
Format:: Supplied as a liquid in 50mM sodium phosphate, pH 7.5, 100mM potassium chloride, 150mM sodium chloride. No preservative added.
Extra Details:: Human CD257 (B cell activating factor, BAFF,TALL-1, Blys, THANK) and CD256 (APRIL, a proliferation inducing ligand) are both type II molecules belonging to the TNF superfamily (TNFSFL #13b and 13 respectively). They are expressed by non-B cells, and are down regulated by mitogenic stimulation (2). BAFF and APRIL bind to at least two receptors: CD267 (TACI, transmembrane activator and CAML-interactor) and CD269 (BCMA, B cell maturation antigen), both of which are restricted to B cells (3,4). Ligation of these receptors with recombinant BAFF dramatically increases IgM production by peripheral blood B cells (1). A third receptor CD268 (BAFFR) is specific for BAFF has also been described (5). BAFF and BAFFR knockout mice have a reduced numbers of mature B cells in the periphery, however TACI and BCMA knockouts do not share this phenotype, suggesting that BAFF-R may the primary receptor for BAFF in mice (8,9,10). Cell surface BAFF can be proteolytically cleaved to form a soluble trimeric molecule (2). Levels of soluble BAFF correspond with levels of autoantibodies in Sjogren's Syndrome (11). Applications: Suitable for use in ELISA and Flow Cytometry. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice