C2549-10A
CD257 (Biotin)
Size
£639.00
- SKU:
- C2549-10A
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant soluble human CD257
- Clone:
- 7H149
- Format:
- Supplied as a liquid in FBS, 50mM Sodium Phosphate pH 7.5, 100mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA, 0.04% NaN3.
- Extra Details:
- .Human CD257 (B cell activating factor, BAFF,TALL-1, Blys, THANK) and CD256 (APRIL, a proliferation inducing ligand) are both type II molecules belonging to the TNF superfamily (TNFSFL #13b and 13 respectively). They are expressed by non-B cells, and are down regulated by mitogenic stimulation(2). BAFF and APRIL bind to at least two receptors: CD267 (TACI, transmembrane activator and CAML-interactor) and CD269 (BCMA, B cell maturation antigen), both of which are restricted to B cells(3,4). Ligation of these receptors with recombinant BAFF dramatically increases IgM production by peripheral blood B cells(1). A third receptor CD268 (BAFFR) is specific for BAFF has also been described(5). BAFF and BAFFR knockout mice have a reduced numbers of mature B cells in the periphery, however TACI and BCMA knockouts do not share this phenotype, suggesting that BAFF-R may the primary receptor for BAFF in mice(8,9,10). Cell surface BAFF can be proteolytically cleaved to form a soluble trimeric molecule(2). Levels of soluble BAFF correspond with levels of autoantibodies in Sjogren's Syndrome(11). Applications: Suitable for use in Flow Cytometry and ELISA. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. Wash 5x10e5 cultured human Raji cells per tube and incubated 45 minutes on ice with 80ul of C2549-10A at 5ug/ml. 2. Cells were washed twice and incubated with secondary reagent Streptavidin/R-PE (S7973-88P) after which they were washed three times, fixed and analyzed by FACS. 3. Cells stained positive with a mean shift of 0.7 log10 fluorescent units when compared to Raji cells with no recombinant CD257 in the primary incubation Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice