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C2548-98H1

CD235ab (Glycophorin A/B, GYPA/B, GPA/B, PAS-2/3, Sialoglycoprotein alpha/beta, GPSAT, GPErik, MSN Blood Group) (FITC)

Size

£607.00

SKU:
C2548-98H1
Application:
Flow Cytometry
Purity:
Purified
Storage Conditions:
-20°C
Supplier:
United States Biological
Host:
Mouse
Immunogen:
Synthetic peptide corresponding to human CD235ab at N-terminal.
Clone:
HIR2
Format:
Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide. Labeled with Fluorescein isothiocyanate (FITC).
Extra Details:
CD235a (glycophorin A, GPA) is a transmembrane sialoglycoprotein expressed on erythrocytes and their precursors. Similarly to CD235b (glycophorin B, GPB), these molecules provide the cells with a large mucin-like surface, which minimalizes aggregation between erythrocytes in the circulation. GPA is the carrier of blood group M and N specificities, while GPB accounts for S, s and U specificities. CD235a is a receptor of Hsa, a Streptococcus adhesin. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 100ul peripheral blood cells or 20ul/10e6 cells. Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Shipping Conditions:
Blue Ice