C2548-98D2
CD235a (Glycophorin A, GpA) (Biotin)
Size
£557.00
- SKU:
- C2548-98D2
- Application:
- Flow Cytometry
- Purity:
- Purified by Protein A affinity chromatography from cell culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Neuraminidase treated human erythrocytes
- Clone:
- 7H140 (A63-B/C2)
- Format:
- Supplied as a liquid in 50mM sodium phosphate pH 7.5, 500mM potassium chloride, 150mM sodium chloride, 0.2% BSA, 0.04% sodium azide, 5% glycerol. Labeled with Biotin.
- Extra Details:
- Human CD235a (glycophorin A) is a sialoglycoprotein expressed early on the cell surface during erythropoiesis as pluripotent stem cells are recruited to differentiate into erythrocytes (1). CD235a (glycophorin A) is a useful marker to study the developmental biology of hematopoietic progenitor cells (2,3). Applications: Suitable for use in Flow Cytometry. Other applications have not been tested. Recommended Dilutions: Flow Cytometry: 10ug/ml Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. 2ul per tube of freshly drawn heparinized human whole blood was washed and incubated for 45 min on ice with 80ul of C2548-98D2 at 10ug/ml. 2. Cells were washed twice and incubated with S7973-88P, Streptavidin/R-Phycoerythrin, after which they were washed three times and fixed. 3. To avoid clots, fixed red blood cells were vortexed and drip-filtered through a 35um nylon mesh filter prior to analysis by FACS. 4. Cells stained positive with a mean shift of 1.35 log10 fluorescent units when compared to a mouse IgM/Biotin negative control at a similar concentration. 5. Binding was blocked when cells were pre-incubated for 10 minutes with 20ul of 0.5mg/ml of C2548-98D1 CD235a (Glycophorin A, GpA). Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice