C2548-97G2

CD226 (CD226 Antigen, Adhesion Glycoprotein, DNAX Accessory Molecule 1, DNAM1, DNAM-1, Platelet and T cell Activation Antigen 1, PTA1, TLiSA1) (Azide Free)

Size

£971.00

SKU:: C2548-97G2
Purity:: Purified by Protein A affinity chromatography from tissue culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Human cytotoxic T lymphocyte clone.
Clone:: 4j67 (DX11)
Format:: Supplied as a liquid in PBS, pH 7.4. No preservative added (Azide free).
Extra Details:: CD226 is broadly expressed on T-cells, NK cells, platelets, monocytes and a subset of B cells. CD226 is also expressed by a subset of CD3 positive thymocytes. C2548-97G2 is reported to inhibit T- and NK cell mediated cytotoxicity against tumor cell targets and to block TNF alpha and IFN gamma secretion by alloantigen-specific T-cells (Kojima et al. 2003). Applications: Suitable for use in Flow Cytometry, Functional Assays and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Flow Cytometry: 1:50-1:200; 10ul labels 1x10e6 cells in 100ul Optimal dilutions to be determined by the researcher. Hybridoma: Sp2/0 myeloma cells with spleen cells from BALB/c mice. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.