C2548-97A-ML490
CD221 (Insulin-like growth factor I Receptor, IGF-I Receptor) (MaxLight 490)
Size
£940.00
- SKU:
- C2548-97A-ML490
- Purity:
- Purified by Protein G affinity chromatography from tissue culture supernatant.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Purified human placental IGF-I receptor.
- Clone:
- 6A396 (1H7)
- Format:
- Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™490.
- Extra Details:
- MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm); Emission (515nm); Extinction Coefficient 73,000. Human CD221 is a 155kD receptor tyrosine kinase, also known as Insulin-like growth factor I receptor (IGF-I Receptor). CD221 is composed of two extracellular alpha-subunits and two transmembrane beta-subunits. CD221 is expressed in a range of tissues, including kidney, liver, placenta, mammary gland, brain, ovary and skin. The ligands for CD221 include IGF-I and IGF-II, which bind to CD221 and activate tyrosine kinase activity, resulting in phosphorylation of several intracellular signalling proteins. Applications: Suitable for use in Flow Cytometry, Western Blot and Immunohistochemistry. Other applications have not been tested. Recommended Dilutions: Flow Cytometry: 1:10. Use 10ul to label 10e6 cells in 100ul. Immunohistochemistry (Paraffin): 1:10-1:40. Protein digestion is required Optimal dilutions to be determined by the researcher. Immunohistochemistry Positive Control: Pancreas, placenta Recommended Negative Control: I1904-78R: IgG1 Murine Negative Control Hybridoma: BALB/c mice were fused with cells of the SP2/0 myeloma cell line Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice