C2548-96W2
CD200 (OX2) (FITC)
Size
£652.00
- SKU:
- C2548-96W2
- Application:
- Flow Cytometry
- Purity:
- Purified by Protein G affinity chromatography from tissue culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rat
- Immunogen:
- Mouse CD200-rat CD4 fusion protein.
- Clone:
- 6A387 (OX-90)
- Format:
- Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.09% sodium azide Labeled with Fluorescein Isothiocyanate (FITC)
- Extra Details:
- CD200, also known as OX2, is expressed by splenic B lymphocytes, follicular dendritic cells, splenic endothelium and by neurons. Recent studies have suggested that the CD200 - CD200 ligand system is of importance in the control of macrophage and granulocyte activation. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 1:5, 10ul labels 10e6 cells in 100ul. The Fc region of monoclonal antibodies may bind non-specifically to cells expressong low afinity Fc receptors. Optimal dilution determined by the researcher Recommended Negative Control: I1904-82E1: IgG2a, Rat Negative Control (FITC) Hybridoma: Spleen cells from immunized rats were fused with cells of the rat Y3 myeloma cell line Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice