C2548-90XA
CD177 (Neutrophil Glycoprotein NB1) (FITC)
Size
£599.00
- SKU:
- C2548-90XA
- Application:
- Flow Cytometry
- Purity:
- Purified by size-exclusion chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Human granulocytes
- Clone:
- 3H1954 (MEM-166)
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide. Labeled with fluorescein isothiocyanate (FITC).
- Extra Details:
- CD177 (NB1/HNA-2a and PRV-1 form) is a GPI-anchored glycoprotein present mainly on neutrophils. Its plasma membrane expression is increased during pregnancy and and inflammation or after G-CSF application. Ligand of CD177 has been identified as CD31 (PECAM-1). CD177 participates in neutrophil transmigration and seems to be also a pro-proliferative molecule. The antibodies against CD177 can be involved in neonatal alloimmune neutropenia (NAN). Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 20ul/100ul of whole blood or 10e6 cells in a suspension. Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice