C2548-90N

CD166 (ALCAM, Activated Leukocyte Cell Adhesion Molecule) (Biotin)

Size

£1,017.00

SKU:: C2548-90N
Purity:: Purified by Protein G affinity chromatography from ascites.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to Trp28-Ala526 from human CD166, expressed in NS0 cells
Clone:: 9H95
Format:: Supplied as a lyophilized powder in PBS with BSA. Reconstitute with sterile 500ul sterile PBS. Labeled with Biotin.
Extra Details:: Human CD166 is a type 1 glycoprotein expressed on activated T cells, B cells and monocytes and appears to be the ligand for CD6 (1,2). Human CD166 may be important for activation of T cells. Applications: Suitable for use in Western Blot and Flow Cytometry. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml under non-reducing conditions only Flow Cytometry: 10ul labels 10e6 cells in human whole blood monocytes Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.