C2548-84N2

CD154 (CD40 Ligand, Cd40l, CD40-L, Cd40lg, sCD40L, HIGM1, IGM, IMD3, Ly62, Ly-62, T-BAM, T-cell Antigen Gp39, TNF-related Activation Protein, TRAP, Tumor Necrosis Factor Ligand Superfamily Member 5, Tnfsf5) (FITC)

Size

£581.00

SKU:: C2548-84N2
Purity:: Purified
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Hamster
Immunogen:: Activated mouse Th1 clone D1.6
Clone:: MR1
Format:: Supplied as a liquid in PBS, 0.09% sodium azide. Labeled with FITC.
Extra Details:: CD154, formerly known as CD40 ligand and gp39, is a type II integral membrane protein and a member of the tumor necrosis factor (TNF) family of ligands.1-7 It is an important accessory molecule in T cell-B cell costimulatory interactions, and is expressed predominantly on activated CD4+ T lymphocytes. It is also present on the surface of activated Th0, Th1, and Th2 T cell clones. Its expression is transient and cyclosporinsensitive.6 Applications: • Flow cytometry 1, 5 • Immunoprecipitation 1 • In vivo and in vitro blockage of CD154 function 1, 3, 5 Recommended Dilution: Flow Cytometry: ≤ 2ug/10e6 cells Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.