C2548-83K

CD152 (CTLA4, Cytolytic T-lymphocyte-associated Antigen 4)

Size

£825.00

SKU:: C2548-83K
Purity:: Purified by affinity chromatography from ascites.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Human CTLA-4/human IgG heavy chain fusion protein.
Clone:: 3H1883 (BNI3)
Format:: Supplied as a liquid in PBS, 0.5% BSA, 0.09% sodium azide.
Extra Details:: CD152 is an inhibitory receptor and negative regulator of T-cell responses. CD152 is a single pass type 1 transmembrane protein belonging to the immunoglobulin superfamily containing a single Ig-v-like domain in the extracellular region. CD152 along with CD28 binds to the co-stimulatory molecules CD80 and CD86 (Azuma et al. 1993). The cytoplasmic domain of CD152 contains a critical tyrosine at residue 201 phosphorylated by Janus Kinase 2 which subsequently controls surface expression through regulation of CD152 interaction with AP-2 (Shiratori et al. 1997, Chikuma et al. 2000). CD152 is expressed primarily as an intracellular antigen with transport to the cell surface under tight regulation of several molecules including Trim, PLD and TIRC7, CD152 also demonstrates rapid internalization once expressed at the cell surface (Valk et al. 2008). CD152 plays a significant role in maintaining tolerance to self antigens and defects in CD152 presentation and expression has been implicated in a number of autoimmune diseases (Romo-Tena et al. 2013). Applications: Suitable for use in Immunohistochemistry, Immunoprecipitation and Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: Neat-1:10. Use 10ul to label 10e6 cells in 100ul. Membrane permeabilisation is required. Immunohistochemistry (Frozen): 5-50ug/ml. The epitope is sensitive to formaldehyde fixation and tissue processing. Acetone fixation is recommended for frozen sections. Not suitable for use on paraffin. Optimal dilution determined by the researcher. Hybridoma: Spleen cells from immunized BALB/c mice were fused with cells of the mouse P3/X63-Ag8.653 myeloma cell line. Recommended Negative Controls: I1904-83P1: IgG2a Mouse Negative Control Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice