C2548-81T
CDw150 (CD150, Signaling Lymphocytic Activation Molecule (SLAM)
Size
£863.00
- SKU:
- C2548-81T
- Purity:
- Purified by Protein G affinity chromatography from hybridoma culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protien corresponding to Ala21-Pro237 from human CDw150, expressed in NS0 cells.
- Clone:
- 9H94
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Extra Details:
- Signaling lymphocytic activation molecule (SLAM, SLAMF1; CD150) was the first identified of a family of type I transmembrane (TM) lymphocyte activating receptors. SLAM homotypic adhesion bidirectionally stimulates T and B cells. SLAM is also expressed by hematopoietic stem cells, dendritic cells and platelets and is a T cell measles virus receptor. The 70kD glycoprotein contains a 216aa extracellular domain (ECD) with one C2 type and one V type Ig-like domain, a 20aa TM sequence and a 76aa SH2-binding cytoplasmic domain. One splice variant has a shorter cytoplasmic tail and another lacks the TM sequence and is secreted. Human and mouse SLAM ECD share 60% aa identity. Applications: Suitable for use in Flow Cytometry and Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 2ug/ml using PMA and ionomycin-treated human peripheral blood mononuclear cells (PBMC). Flow Cytometry: 2.5ug/10e6 cells using human peripheral blood lymphocytes Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice