C2548-81K

CD148 (Receptor-type Tyrosine-protein Phosphatase eta, Protein-tyrosine Phosphatase eta, R-PTP-eta, Density-enhanced Phosphatase 1, DEP-1, HPTP eta, Protein-tyrosine Phosphatase Receptor Type J, R-PTP-J, PTPRJ, DEP1)

Size

£351.00

SKU:: C2548-81K
Application:: Flow Cytometry
Purity:: Purified by affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: PHA-stimulated human PBMC.
Clone:: A3
Format:: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Extra Details:: CD148, a receptor-like protein tyrosine phosphatase also known as human protein tyrosine phosphatase-eta (HPTP-eta) or density-enhanced protein tyrosine phosphatase-1 (DEP-1), is involved in signal transduction in leukocytes and is thought to contribute to mechanisms of cellular differentiation. In lymphoid organs, CD148 was found to be widely expressed on B and T cells, granulocytes, macrophages, certain dendritic cells as well as mature thymocytes. The cellular level of CD148 was increased after in vitro activation of peripheral blood leukocytes. CD148 as a leukocyte activation marker and may be involved in the regulation of T cell activation. Leukocytes expressing CD148 are significantly upregulated in inflamed tissues and that a subset of these cells co-expresses the activation marker CD25. In non-lymphoid tissues, CD148 was found to be present on many epithelial cell types with glandular and/or endocrine differentiation as well as on fibrocytes, melanocytes and Schwann cells. Among non-hematopoietic cells, CD148 is expressed by characteristic types of epithelial and non-epithelial cells. Downregulation of CD148 might promote dedifferentiation and autonomous growth of such cells in malignant tumors. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: ≤1ug/10e6 cells in 100ul volume or whole blood Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice