C2548-79

CD146 (Mucin 18, MUC18 Glycoprotein, Melanoma Associated Antigen A32, S-endo 1 Endothelial Associated Antigen, Melanoma Adhesion Molecule Mel-CM)

Size

£665.00

SKU:: C2548-79
Purity:: Purified by Protein A affinity chromatography from tissue culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to human MUC18 (D1-D5) Fc.
Clone:: 3H1869 (OJ79c)
Format:: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Extra Details:: CD146 is a member of the immunoglobulin superfamily. It is expressed by all endothelial cells and by melanoma cells. CD146 appears to act as an adhesion molecule. Expression in melanoma may be linked to tumor progression. Applications: Suitable for use in Flow Cytometry, ELISA. Immunofluorescence and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 1:10-1:50. 10ul labels 10e6 cells in 100ul. Immunohistochemistry: Frozen Optimal dilutions to be determined by the researcher. Immunohistochemistry Recommended Positive Control Tissue: Melanoma Hybridoma: Spleen cells from immunized mice were fused with cells of the mouse Sp2/0 Ag.14 myeloma cell line. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.