C2548-63C
CD138 (Syndecan 1)
Size
£492.00
- SKU:
- C2548-63C
- Purity:
- Supernatant
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- U-266 cells. Epitope: Ectodomain; Cellular Localization: Cell membrane. MW of Antigen: 30.5 (core protein); 85-92kD (depending on glycosaminoglycans).
- Clone:
- 5G71
- Format:
- Supplied as a liquid, 0.09% sodium azide
- Extra Details:
- CD138/Syndecan-1 is a transmembrane heparin sulfate proteoglycan which is made up of one core protein and five glycosaminoglycan. CD138 is expected to play a role in cell adhesion. It is expressed on the surface of pre- B cells and plasma cells but is absent from mature B cells. It is a selective marker for B cell lymphoblastic leukemia and lympho plasmocytoid leukemia. It is lost from the apoptotic myeloma cells; hence is a useful marker for viable myeloma cells. Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation, and Immunohistochemistry. Other applications have not been tested. Recommended Dilutions: Immunohistochemistry (Formalin/paraffin): 1:10-1:20 for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilutions to be determined by the researcher. Positive Control: U-266 cells, Tonsil. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice