C2548-61
CD138 (Syndecan 1)
Size
£550.00
- SKU:
- C2548-61
- Purity:
- Purified by Protein G affinity chromatography from tissue culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- U266 cell line
- Clone:
- B-A38
- Format:
- Supplied as a liquid in PBS, 1% BSA, 0.09% sodium azide.
- Extra Details:
- CD138 (Syndecan-1) is a heparan sulfate rich membrane glycoprotein of 200kD. Syndecan functions as a receptor for collagen, fibronectin and thrombospondin. CD138 consists of one core protein and five glycosaminoglycan. CD138 is expected to play a role in cell adhesion. It is expressed on the surface of pre-B cells and plasma cells but is absent from mature B cells. It is a selective marker for B cell lymphoblastic leukemia and lympho plasmocytoid leukemia. It is lost from the apoptotic myeloma cells; hence is a useful marker for viable myeloma cells. Applications: Suitable for use in ELISA, Flow Cytometry, Immunofluorescence and Immunohistochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: Neat. 10ul labels 10e6 cells in 100ul. Immunohistochemistry (Frozen, paraffin): 1:100-1:500. Requires heat pre-treatment. Sodium citrate buffer, pH 6.0 is recommended for this purpose. Optimal dilutions to be determined by the researcher. Immunohistochemistry Positive Control: Bone Marrow Hybridoma: X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice