C2548-39K

CD137 (4-1BB, TNFRSF9, 4-1BB ligand receptor, CD137, CD137 antigen, CDw137, ILA, MGC2172, T-cell antigen 4-1BB homolog, T-cell antigen ILA, Tumor necrosis factor receptor superfamily member 9 precursor) (FITC)

Size

£639.00

SKU:: C2548-39K
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: 4°C (-20°C Glycerol)
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Ectodomain of recombinant human 4-1BB fusion protein
Clone:: 7H126
Format:: Supplied as a liquid in PBS, 0.2% BSA, 0.04% sodium azide, 5% glycerol.
Extra Details:: Human CD137 (4-1BB) is expressed on activated T cells within 24-48 hours of activation. CD137 is a type I membrane protein and a member of the tumor necrosis factor receptor superfamily. CD137 appears to be important for T cell proliferation and survival. Applications: Suitable for use in Flow Cytometry and FLISA. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:50, 80ul/test Optimal dilutions to be determined by the researcher. Functional Application: Antibody blocks binding of CD137 huIg to Raji cells Fluorescein/IgG Molar Ratio: 9.8 Performance: Cultured human CEM were stimulated 2 days in the presence of PMA (10ng/ml) and ionomycin (1mM). 5 x 10e5 cells were washed and incubated 45 minutes on ice with 80ul of C2548-39K, 1:50 Cells were washed three times, fixed and analyzed on a BD FACstar plus. Cells stained positive with a mean shift of 1.03 log10 fluorescent units when compared to a Mouse IgG1/FITC negative control at a similar concentration. Binding was 85% blocked when cells were pre-incubated 10 minutes with 20ul of 0.5mg/ml anti-CD137 antibody C2548-39F. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice