C2548-39F
CD137 (4-1BB, TNFRSF9, 4-1BB ligand receptor, CD137, CD137 antigen, CDw137, ILA, MGC2172, T-cell antigen 4-1BB homolog, T-cell antigen ILA, Tumor necrosis factor receptor superfamily member 9 precursor)
Size
£557.00
- SKU:
- C2548-39F
- Purity:
- Purified by Protein A affinity chromatography from tissue culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Ectodomain of recombinant human 4-1BB fusion protein.
- Clone:
- 7H126
- Format:
- Supplied as a liquid in 50mM sodium phosphate, pH 7.5, 100mM potassium chloride, 150mM sodium chloride, 0.5mg/ml gentamicin sulfate
- Extra Details:
- Human CD137 (4-1BB) is expressed on activated T cells within 24-48 hours of activation. CD137 is a type I membrane protein and a member of the tumor necrosis factor receptor superfamily. CD137 appears to be important for T cell proliferation and survival. Applications: Suitable for use in Flow Cytometry and ELISA Other applications not tested. Recommended Dilutions: Flow Cytometry: 10ug/ml. 80ul labels 5x10e5 cultured human CEM cells. Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. Cultured human CEM were stimulated 1 day in the presence of PMA (10ng/ml) and ionomycin (1uM). 2. Five x 10e5 cells per tube were washed and incubated 45 minutes on ice with 80ul of C2548-39F at 10ug/ml. 3. Cells were washed twice and incubated with IgG (FITC) goat anti-mouse, after which they were washed three times, fixed and analyzed by FACS. 4. A 38% subpopulation of the cells stained positive with a mean shift of 1.14 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice