C2515-40D

CD134 Ligand (CD134L, OX40L, CD252) (PE)

Size

£735.00

SKU:: C2515-40D
Purity:: Purified by Protein A affinity chromatography from tissue culture supernatant.
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Human HUVEC and recombinant soluble human CD252.
Clone:: 7H146
Format:: Supplied as a liquid in 50mM sodium phosphate, pH 7.5, 500mM potassium chloride, 150mM sodium chloride, 0.2% BSA, 0.04% sodium azide, 15% glycerol. Labeled with R-Phycoerythrin (PE).
Extra Details:: Human CD134L is a type II membrane protein with homology to TNF which is expressed on activated B cells and activated endothelial cells. CD134L binds to CD134 present on activated T cells, providing a costimulatory signal. This pathway seems to be more effective for costimulation of CD4+ helper T cells than for CD8+ effector T cells. Blockade of this interaction in mouse abrogated immunological effects in several models of immflamation and rejection. Applications: Suitable for use in Flow Cytometry and ELISA. Other applications not tested. Recommended Dilutions: Flow Cytometry: 10ug/ml (1:50) labels 5x10e5 HUVEC cells Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. Cultured human umbilical cord vein endothelial cells (HUVEC) were harvested by trypsinization. 2. Five x 10e5 cells per tube were washed and incubated for 45 minutes on ice with 80ul of C2515-40D at 10ug/ml (1:50). 3. Cells were washed three times, fixed and analyzed by FACS. 4. Cell stained positive with a mean shift of 0.89log10 fluorescent units when compared to a Mouse IgG1/R-PE at a similar concentration. 5. Binding was blocked when cells were pre incubated for 10 minutes with 20ul of C2515-40A (0.5mg/ml). Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

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