C2515-40C
CD134 Ligand (CD134L, OX40L, CD252) (FITC)
Size
£672.00
- SKU:
- C2515-40C
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- 4°C (-20°C Glycerol)
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Human HUVEC and Recombinant soluble human CD134L
- Clone:
- 7H146
- Format:
- Supplied as a liquid in 50mM sodium phosphate pH 7.5, 100mM potassium chloride, 150mM sodium chloride, 0.2% BSA, 0.04% sodium azide, 5% glycerol. Labeled with FITC. Molar Ratio: 8.1 FITC /IgG.
- Extra Details:
- Human CD134L (OX-40 Ligand) is a type II membrane protein with homology to TNF which is expressed on activated B cells and activated endothelial cells. CD134L binds to CD134 present on activated T cells, providing a costimulatory signal (1). This pathway seems to be more effective for costimulation of CD4+ helper T cells than for CD8+ effector T cells (4). Blockade of this interaction in mouse abrogated immunological effects in several models of immflamation and rejection (5,6,7,8). Applications: Suitable for use in Flow Cytometry, FLISA and blocking studies. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:50 (or 1.6ul neat/5x10e5 cells/test) Blocking: Blocks binding of Recombinant CD134 to CD134L on HUVEC. Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. Cultured human umbilical cord vein endothelial cells (HUVEC) were harvested by trypsinization. 5x10e5 cells per tube were washed and incubated 45 minutes on ice with 80ul of C2515-40C at a 1:50 dilution (10ug/ml). 2. Washed three times, fixed and analyzed on a BD FACS Calibur A. Binding was 87% blocked when cells were pre-incubated 10 minutes with 20ul of 0.5mg/ml C2515-40A. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice