C2515-40AX
CD134 Ligand (CD134L, CD252, Glycoprotein Gp34, GP34, OX40 Ligand, OX40L, OX-40L, TAX Transcriptionally-activated Glycoprotein 1, TXGP1, Tumor Necrosis Factor Ligand Superfamily Member 4, TNFSF4)
Size
£557.00
- SKU:
- C2515-40AX
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Human HUVEC and recombinant protein corresponding to soluble human CD134 Ligand
- Clone:
- 7H146
- Format:
- Supplied as a liquid in PBS. No preservative added.
- Extra Details:
- Human CD134L (OX-40 Ligand) is a type II membrane protein with homology to TNF which is expressed on activated B cells and activated endothelial cells. CD134L binds to CD134 present on activated T cells, providing a costimulatory signal. This pathway seems to be more effective for costimulation of CD4+ helper T cells than for CD8+ effector T cell. Blockade of this interaction in mouse abrogated immunological effects in several models of immflamation and rejection. Applications: Suitable for use in ELISA and Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 10mg/ml Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. Cultured human umbilical cord vein endothelial cells were harvested by trypsinization, after which they were washed and incubated 45 minutes on ice with 80ul of C2515-40AX at 10mg/ml. 2. Cells were washed twice and incubated with secondary reagent Goat anti-Mouse IgG (FITC), after which they were washed three times, fixed and analyzed by FACS. 3. A net 14% subpopulation of the cells stained positive with a mean shift of 1.0 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice