C2515-40A
CD134 Ligand (CD134L, OX40L, CD252)
Size
£557.00
- SKU:
- C2515-40A
- Purity:
- Purified by Protein A affinity chromatographyfrom tissue culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Human HUVEC and recombinant protein corresponding to soluble human CD134 Ligand.
- Clone:
- 7H146
- Format:
- Supplied as a liquid in 50mM sodium phosphate pH 7.5, 100mM potassium chloride, 150mM sodium chloride, 0.5mg/ml gentamicin sulfate.
- Extra Details:
- Human CD134L (OX-40 Ligand) is a type II membrane protein with homology to TNF which is expressed on activated B cells and activated endothelial cells. CD134L binds to CD134 present on activated T cells, providing a costimulatory signal (1). This pathway seems to be more effective for costimulation of CD4+ helper T cells than for CD8+ effector T cells (4). Blockade of this interaction in mouse abrogated immunological effects in several models of immflamation and rejection (5,6,7,8). Applications: Suitable for use in Flow Cytometry and ELISA. Other applications not tested. Recommended Dilutions: Flow Cytometry: 10ug/ml Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. Cultured human umbilical cord vein endothelial cells were harvested by trypsinization, after which they were washed and incubated 45 minutes on ice with 80ul of C2515-40A at 10ug/ml. 2. Cells were washed twice and incubated with secondary reagent goat anti-mouse IgG (FITC), after which they were washed three times, fixed and analyzed by FACS. 3. A net 40% subpopulation of the cells stained positive with a mean shift of 0.65 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice