C2515-20K
CD134 (OX40) (FITC)
Size
£633.00
- SKU:
- C2515-20K
- Application:
- Flow Cytometry
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Phytohemagglutinin (PHA) activated Rat Lymph Node Cells.
- Clone:
- OX-40
- Format:
- Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide. Labeled with Fluorescein isothiocyanate (FITC).
- Extra Details:
- CD134, also known as OX40, is a 43kD type I membrane protein and member of the tumor necrosis factor receptor superfamily, expressed predominantly by CD4+ activated T cells, and a key regulator of T cell-dependent immune responses. CD134 has been identified as the receptor for the OX40 ligand, CD252, expressed by activated B cells, acting as a co-stimulatory signal for the stimulation and secretion of immunoglobulins. CD134 has also been identified as a binding receptor for Feline Immunodeficiency Virus (FIV), acting along with CXC chemokine receptor 4 (CXCR4) to facilitate the entry of the virus into CD4+ primary target cells. Fusion Partners: Spleen cells from immunized BALB/c mice were fused with cells from the NS0/1 Ag4.1 mouse myeloma cell line. FITC: Protein (molar ratio): 5.6: 1 Recommended Negative Controls: Mouse IgG2b Negative Control: FITC Applications: Flow Cytometry: Neat Optimal working dilutions to be determined by researcher. Flow Cytometry: Use 10ul of the suggested working dilution to label 10e6 cells in 100ul. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice