C2515-19C
CD134 (OX40) (PE)
Size
£735.00
- SKU:
- C2515-19C
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Human HUT 102 T cells
- Clone:
- 7H163
- Format:
- Supplied as a liquid in 50mM sodium phosphate pH 7.5, 500mM potassium chloride, 150mM sodium chloride, 0.2% BSA, 0.04% sodium azide, 15% Glycerol. Labeled with R-Phycoerythrin (PE).
- Extra Details:
- Human CD134 (OX40) is an activation-associated antigen which is predominantly expressed on activated CD4 positive cells. CD134 antigen is a member of the tumor necrosis factor (TNF) receptor family of molecules and may be involved with regulating T cell-dependent B cell proliferation and differentiation. Applications: Suitable for use in Flow Cytometry, Immunohistochemistry and FLISA. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:50 (5ug/ml) labels 5x10e5 cultured HPB-MLT human tumor cells Optimal dilutions to be determined by the researcher. Flow cytometry Protocol: 1. Five x 10e5 cultured HPB-MLT human tumor cells were washed and incubated 45 minutes on ice with 80ul of C2515-19C at a 5ug/ml). 2. Cells were washed three times, fixed and analyzed by FACS. 3. Cells stained positive with a mean shift of 0.85 log10 fluorescent units when compared to a Mouse IgG1/R-PE negative control at a similar concentration. Binding was blocked when cells were pre incubated 10 minutes with 20ul of 0.5mg/ml anti-CD134 antibody (C2515-19). Storage and Stability: May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months at 4°C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.
- Shipping Conditions:
- Blue Ice