C2515-19B
CD134 (OX40) (FITC)
Size
£639.00
- SKU:
- C2515-19B
- Purity:
- Purified by Protein A affinity chromatography fro tissue culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Human HUT 102 T cells
- Clone:
- 7H163
- Format:
- Supplied as a liquid in 50mM sodium phosphate pH 7.5, 100mM potassium chloride, 150mM sodium chloride, 5% glycerol, 0.2% BSA, 0.04% sodium azide. Labeled by Fluorescein isothiocyanate (FITC)
- Extra Details:
- Human CD134 (OX40) is an activation-associated antigen which is predominantly expressed on activated CD4 positive cells. CD134 antigen is a member of the tumor necrosis factor (TNF) receptor family of molecules and may be involved with regulating T cell-dependent B cell proliferation and differentiation. Applications: Suitable for use in Flow Cytometry, ELISA, Immunohistochemistry, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:50 (10ug/ml) Immunohistochemistry: Fixed cells or tissues Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. 5x10e5 cultured HPB-MLT cells were washed and incubated 45 minutes on ice with 80ul of C2515-19B at 1:50 (10ug/ml).. 2. Cells were washed three times and fixed and analyzed by FACS. 3. Cells stained positive with a mean shift of 0.9 log10 fluorescent units when compared to a Mouse IgG1/FITC Negative Control at a similar concentration. 4. Binding was blocked when cells were preincubated 10 minutes with 20ul of 0.5mg/ml C2515-19. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice