C2464-01
CD126 (Interleukin 6 Receptor, IL-6R)
Size
£550.00
- SKU:
- C2464-01
- Purity:
- Purified by Protein A affinity chromatography from tissue culture supernatant
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- CHO cells transfected with cDNA of the IL-6 receptor
- Clone:
- 3H1836 (B-R6)
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide
- Extra Details:
- The IL-6 receptor system consists of two chains, IL-6R alpha and gp130. The IL-6R alpha is the binding component specific to the IL-6, but does not transduce a signal alone. On the contrary, gp130 transmits signals not only of IL-6, but also IL-6 related cytokines, i.e. LIF, OSM, CNTF, IL-11 and CT-1. The low affinity binding sites for IL-6, is composed of IL-6R alpha alone. At low concentrations, soluble IL-6R alpha acts as an agonist of IL-6 activity. Applications: Suitable for use in Flow Cytometry, Immunohistochemistry, ELISA, Immunoprecipitation, Inhibition assays, Functional assays and Immunofluorescence. Other applications not tested. Recommended Dilutions: Flow Cytometry: 1:50 Use 10ul to label 10e6 cells in 100ul. Immunohistochemistry: Frozen sections Functional assays: Removal of sodium azide is recommended Optimal dilutions to be determined by the researcher. Hybridoma: Spleen cells from immunized BALB/c (Iffa Credo) mice were fused with the X63/Ag.8653 mouse myeloma cell line. Recommended Secondary Reagents: I1904-66A: IgG, F(ab’)2, X-Adsorbed (FITC) Recommended Negative Controls: I1904-78R: IgG1 Murine Negative Control Storage and Stability:: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice