C2460-06C
CD122 (CD122 Antigen, High Affinity IL-2 Receptor beta Subunit, Interleukin 2 Receptor beta, Interleukin 2 Receptor Subunit beta, IL-2 Receptor, IL-2RB, IL2RB, OTTHUMP00000028799, P70-75, p75) (Biotin)
Size
£435.00
- SKU:
- C2460-06C
- Purity:
- Purified.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rat
- Immunogen:
- Rat myeloma YB2/0 transfected with truncated mouse IL-2RB cDNA.
- Clone:
- 5H4
- Format:
- Supplied as a liquid in PBS, 0.09% sodium azide. Labeled with Biotin.
- Extra Details:
- The IL-2 receptor is a complex of three distinct polypeptide chains: (i) the alpha chain which binds IL-2 with low affinity; (ii) the beta chain that binds IL-2 with high affinity; and (ii) the common gamma chain (gC) that does not bind IL-2.2 The high affinity receptor complex is an alpha/beta/gamma heterotrimer with a Kd of 1.3 x 10-11 M.2 In mouse spleen, CD122 is expresed on ≈ 30% of CD8+ cells and all NK cells, but < 1% of B cells and CD4+ T lymphocytes. In the thymus, its expression is confined to CD4-CD8+ single positive and CD4-CD8-double negative cells.4 Cytoplasmic regions of the IL-2R beta chain are involved in IL-2-mediated cellular signaling and, via the interaction of IL-2 and its receptor complex, may be involved in the generation and differentiation of T lymphocytes.1, 4 Applications: • Flow cytometry 1 • Immunoprecipitation 1 Recommended Dilution: Flow Cytometry: ≤ 1ug/10e6 cells Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice