C2460-01F
CD122 (CD122 Antigen, High Affinity IL-2 Receptor beta Subunit, Interleukin 2 Receptor beta, Interleukin 2 Receptor Subunit beta, IL-2 Receptor, IL-2RB, IL2RB, OTTHUMP00000028799, P70-75, p75)
Size
£479.00
- SKU:
- C2460-01F
- Application:
- Flow Cytometry
- Purity:
- Purified by Protein A affinity chromatography from tissue culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- YT-1 acute human leukemia cells.
- Clone:
- 7H139
- Format:
- Supplied as a liquid in 50mM sodium phosphate, pH 7.5, 100mM potassium chloride, 150mM sodium chloride, 0.5 mg/ml Gentamicin Sulfate.
- Extra Details:
- Human CD122 (IL-2 R beta) associates with CD25 (IL-2R alpha) to form a high affinity receptor for IL-2 and is expressed on activated T cells, B cells and monocytes. The cytoplasmic domain of CD122 is involved with signal transduction. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 20ug/ml. 80ul labels 5x10e5 ficoll prepared human peripheral blood lymphocytes. Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. Five x 10e5 ficoll prepared human peripheral blood lymphocytes were stimulated by incubating 5 days with 5ug/ml PHA-P (P3370-61). 2. Stimulated cells were harvested and incubated 45 minutes on ice with 80ul of C2460-01F at 20ug/ml. 3. Cells were washed twice and incubated with I1904-04E: IgG Pab Goat x Mouse (FITC), after which they were washed three times, fixed and analyzed by FACS. 4. Cells stained positive with a mean shift of 0.17 log10 fluorescent units when compared to a Mouse IgG2a negative control at a similar concentration. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice