C2448-05D
CD117 (c-kit, Stem Cell Factor, SCF Receptor, Steel Factor Receptor, Mast Cell Growth Factor, MGF)
Size
£659.00
- SKU:
- C2448-05D
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to the cytoplasmic domain of human CD117/c-kit. Epitope: Cytoplasmic domain. Cellular Localization: Cell membrane. MW of Antigen: 145kD
- Format:
- Supplied as a liquid in 10mM PBS, pH 7.6, 0.2% BSA, 15mM sodium azide.
- Extra Details:
- Recognizes a protein of 145kD, which is identified as CD117/p145kit. This rabbit polyclonal antibody does not interfere with the binding of SCF to c-kit. It precipitates both the unoccupied as well as the occupied form of c-kit. The binding of the stem cell factor (SCF) to the c-kit-encoded receptor tyrosine kinase (Type III) stimulates a variety of biochemical responses that culminate in cellular proliferation, migration, or survival. C-kit plays an important role in hematopoiesis, melanogenesis, and gametogenesis Applications: Suitable for use in Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry (Formalin/paraffin): 1:100-1:200 for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilutions to be determined by the research Positive Control: Stromal tumor, skin or tonsil. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice