C2448-04E
CD117, phosphorylated (Tyr719) (c-kit, Stem Cell Factor, SCF Receptor, Steel Factor Receptor, Mast Cell Growth Factor, MGF)
Size
£735.00
- SKU:
- C2448-04E
- Purity:
- Purified
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide (SDSTNEYpMDMKPG) derived from the human phosphorylated c-kit (Tyr719) (KLH).
- Format:
- Supplied as a lyophilized powder from 0.01M PBS, pH 7.4. Reconstitute with 100ul of sterile ddH2O.
- Extra Details:
- c Kit, also known as CD117 and stem cell factor receptor, is a 145kD transmembrane tyrosine kinase encoded by the c Kit proto-oncogene. c Kit acts to regulate a variety of biological responses including cell proliferation, apoptosis, chemotaxis and adhesion. Ligand binding to the extracellular domain leads to autophosphorylation on several tyrosine residues within the cytoplasmic domain, and activation. c Kit mutations correlate with tumor growth and progression in a variety of cancers including mast cell disease, gastrointestinal stromal tumor, acute myeloid leukemia, Ewing sarcoma, and lung cancer. Phosphorylation at tyrosine 936 of c Kit allows binding of Grb2 and activation of the Ras, Raf, ERK1&2 signaling pathway. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilutions: ELISA: 1:64,000, peptide coated at 100ng/well. Other applications not tested. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute by adding sterile 40-50% glycerol, ddH2O. Aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice