C2448-03M
CD117 (c-kit, Stem Cell Factor, SCF Receptor, Steel Factor Receptor, Mast Cell Growth Factor, MGF)
Size
£479.00
- SKU:
- C2448-03M
- Application:
- Flow Cytometry
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Human MO7e tumor cells.
- Clone:
- 7H131
- Format:
- Supplied as a liquid in PBS, 0.5mg/ml gentamicin sulfate.
- Extra Details:
- Human CD117 (c-kit) is a transmembrane protein with receptor tyrosine kinase capacity and serves as the receptor for steel factor (SLF). CD117 is expressed on almost all hematopoietic progenitor cells and receptor/ligand interaction is crucial for development of hematopoietic stem cells. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 5ug/ml labels 5x10e5 cultured HEL92.1.7 cells Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. Five x 10e5 cultured HEL92.1.7 cells were washed and incubated 45 minutes on ice with 80ul of C2448-03M at a concentration of 5ug/ml. 2. Cells were washed two times and incubated with I1904-06F: IgG, H&L (FITC) (X-Adsorbed) Pab Gt xMo 3. Washed three times, fixed and analyzed by FACS. 4. Cells stained positive with a mean shift of 0.76 log10 fluorescent units when compared to a I1904-78R: IgG1 Murine Negative Control at a similar concentration. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice