C2448-03K
CD117 (c-kit, Stem Cell Factor, SCF Receptor, Steel Factor Receptor, Mast Cell Growth Factor, MGF) (PE)
Size
£704.00
- SKU:
- C2448-03K
- Application:
- Flow Cytometry
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Human MO7e tumor cells.
- Clone:
- 7H131
- Format:
- Supplied as a liquid in PBS, 0.2% BSA, 0.04% sodium azide, 15% glycerol. Labeled with R-Phycoerythrin (PE).
- Extra Details:
- Human CD117 (c-kit) is a transmembrane protein with receptor tyrosine kinase capacity and serves as the receptor for steel factor (SLF). CD117 is expressed on almost all hematopoietic progenitor cells and receptor/ligand interaction is crucial for development of hematopoietic stem cells. Applications: Suitable for use in Flow Cytometry. Does not block binding of SCF. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:50, 80ul/test Optimal dilutions to be determined by the researcher. Protocol: Five x 10e5 cultured HEL human tumor cells were washed and incubated 45 minutes on ice with 80ul of C2448-03K at a 1:50 dilution. Cells were washed three times, fixed and analyzed. Cells stained positive with a mean shift of 1.4 log10 fluorescent units when compared to a mouse IgG1/PE negative control at a similar concentration. Binding was 93% blocked when cells were pre incubated 10 minutes with 20ul of 0.5mg/ml C2448-03K. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
- Shipping Conditions:
- Blue Ice