C2446-50K3
CD105 (Endoglin, gp160, TGF beta1,3 Receptor) (PE)
Size
£741.00
- SKU:
- C2446-50K3
- Application:
- Flow Cytometry
- Purity:
- Purified by Protein G affinity chromatography from cell culture supernatant.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to Glu26-Gly586 from human CD105, expressed in NS0 cells.
- Clone:
- 8H109
- Format:
- Supplied as a liquid in saline, 0.5% BSA, 0.09% sodium azide. Labeled with R-Phycoerythrin (PE).
- Extra Details:
- CD105, also known as Endoglin, is a disulfide-linked homodimeric type I transmembrane glycoprotein (1, 2). Two isoforms of CD105, a short form and a long form, differ by 33 aa in their cytoplasmic tails (3). The long form (180kD in size) is considered the predominant form of the protein although both isoforms have been detected by RT-PCR in endothelial cells, monocytic cell lines, and placenta (3, 4). CD105 serves as the regulatory component of the TGF-β receptor complex by associating with TGF-β receptor I and II (5, 6). These receptor complexes efficiently bind TGF-β1 and TGF-β3 with high affinity (5, 7). CD105 also binds other members of the TGF-β superfamily including activin-A, BMP-7 and BMP-2 (5). Functionally, CD105 plays an important role in angiogenesis (7, 8), hematopoiesis (9, 10) and cardiac development (9). CD105 was also shown to be a functional marker that defines long-term repopulating hematopoietic stem cells from the bone marrow "side-population" cells (11). Multipotent stem cells have been isolated from bone marrow and muscle tissue based on their expression of CD105 (12, 13). Clinically, mutations in the gene for CD105 result in the autosomal dominant disorder Hereditary Hemorrhagic Telangiectasia Type 1 (14). In addition, soluble levels of shed CD105 have been associated with poor prognosis in breast, colorectal and other types of cancer (15, 16). Further investigations may elucidate the role of CD105 and its ligands in a variety of biologic processes. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: Use 10ul to label 10e6 cells in U937 human cell line Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
- Shipping Conditions:
- Blue Ice