C2446-50E1
CD105 (Endoglin, gp160, TGF beta1,3 Receptor) (Biotin)
Size
£557.00
- SKU:
- C2446-50E1
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Membrane preparation of human B-lineage leukemia cells
- Clone:
- SN6/N1-3A1
- Format:
- Supplied as a liquid in PBS, pH 7.5, 5% Glycerol, 0.2% BSA, 0.04% and 0.04% sodium azide.. Labeled with biotin.
- Extra Details:
- Human CD105 (endoglin) is a homodimeric membrane glycoprotein virtually restricted to endothelial cells, and present on some leukemia cells and minor subsets of bone marrow cells. CD105 binds to TGF-b1 and b3 with high affinity. Antibody will augment binding of TGF-b1 to CD105 expressing leukemia cells Applications: Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. 5x10e5 Nalm-6 were washed and incubated 45 minutes on ice with 80ul of C2446-50E1 at 5ug/ml. 2. Cells were washed twice and incubated with secondary reagent S7973-88P, Streptavidin/R-Phycoerythrin, after which they were washed three times fixed and analyzed using a FACS. 3. Cells stained positive with a mean shift of 1.81 log10 fluorescent units when compared to I1904-78R2 IgG1/biotin Murine Negative Control at a similar concentration. 4. Binding was 99% blocked when cells were preincubated 10 minutes with 20ul of 0.5mg/ml C2446-50E, CD105. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice