C2446-50E
CD105 (Endoglin, gp160, TGF beta1,3 Receptor)
Size
£479.00
- SKU:
- C2446-50E
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Membrane preparation of human B-lineage leukemia cells
- Clone:
- SN6/N1-3A1
- Format:
- Supplied as a liquid in PBS, pH 7.5, 0.5mg/ml Gentamicin Sulfate.
- Extra Details:
- Human CD105 (endoglin) is a homodimeric membrane glycoprotein virtually restricted to endothelial cells, and present on some leukemia cells and minor subsets of bone marrow cells. CD105 binds to TGF-b1 and b3 with high affinity. Applications: Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested. Recommended Dilution: Flow cytometry: 10ug/ml Immunohistochemistry: frozen sections Optimal dilutions to be determined by the researcher. Flow cyytometry Protocol: 1. Five x 10e5 cultured Nalm-6 human tumor cells were washed and incubated 45 minutes on ice with 80ul of C2446-50E at a concentration of 10ug/ml. 2. Cells were washed twice and incubated with secondary reagent Goat anti-Mouse IgG/FITC 3. After which they were washed three times, fixed and analyzed by FACS. 4. Cells stained positive with a mean shift of 0.79 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice