C2445-65E
CD103 (CD103 Antigen, HML-1 Antigen, Human Mucosal Lymphocyte Antigen 1, Huminae, Integrin alpha E, ITGAE, Integrin alpha IEL, MGC141996, Mucosal Lymphocyte 1 Antigen, Mucosal Lymphocyte Antigen 1) (Biotin)
Size
£531.00
- SKU:
- C2445-65E
- Application:
- Flow Cytometry
- Purity:
- Purified
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Hamster
- Immunogen:
- C57BL/6J mouse intestinal intraepithelial lymphocytes
- Clone:
- 2E7
- Format:
- Supplied as a liquid in PBS, 0.09% sodium azide. Labeled with Biotin.
- Extra Details:
- CD103 is a member of the integrin series of adhesion molecules. This antigen defines a developmentally important subset of T cells, namely mucosal T cells including all IEL (intraepithelial lymphocytes) and ~20% of lamina propria T cells. Expression of CD103 is more restricted outside these mucosal organs, appearing at lower levels on T cell subsets of the lymph node, dendritic epidermis and periphery In non-epithelial CD103+ T cells there is a bias toward expression on CD8+ cells. This antibody is reported to have signal-inducing activity in a redirected lysis assay and to co-stimulate IEL and CD8+ lymph node cells in conjunction with anti-TCR in vitro, which runs counter to the usual inhibition of CTL by anti-integrins. The property of costimulation is not unique to the mouse since the combination of anti-human alphaIEL plus anti-TCR will produce similar proliferation in human cells, with the exception that CD8+ cells are not stimulated. Applications: Suitable for use in Flow Cytometry. Other applications have not been tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice