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C2445-30C

CD101 (EWI-101, Glu-Trp-Ile EWI motif-containing protein 101, Gm1016, Gm734, Igsf2, IgSF2, Immunoglobulin superfamily member 2, LOC381460)

Size

£863.00

SKU:
C2445-30C
Application:
Flow Cytometry
Purity:
Purified by Protein G affinity chromatography from hybridoma culture supernatent
Storage Conditions:
-20°C
Supplier:
United States Biological
Host:
Rat
Immunogen:
Recombinant protein corresponding to Gln21-Tyr1033 from mouse CD101 expressed in CHO cells.
Clone:
7H22
Format:
Supplied as a lyophilized powder from a 0.2um filtered solution in PBS, pH 7.4 with 5% trehalose. Reconstitute with 200ul sterile PBS
Extra Details:
IGSF2, also known as CD101, is an Ig-superfamily transmembrane protein. It is expressed on dendritic cells, Langerhans cells, granulocytes, and activated T cells. IGSF2 ligation is involved in T cell activation. Polymorphisms in IGSF2 are associated with susceptibility to type I diabetes. The extracellular domains of mouse and human IGSF2 share 70% amino acid sequence identity. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 2.5ug/10e6 cells. Detects CD101 in mouse peripheral blood GR-1+ granulocytes. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Shipping Conditions:
Blue Ice