CD99 (MIC2, p30/32MIC2, Ewing’s Sarcoma Marker, E2 Antigen)

Size

£882.00

SKU:: C2444-12E
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: Recombinant protein corresponding to aa23-122 at the extracellular domain of human CD99, expressed in NS0 cells.
Format:: Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Extra Details:: Human CD99 (also known as MIC2 and E2 antigen) is a 32kD Type I, transmembrane glycoprotein expressed in T cells, CD34+ stem cells, monocytes and NK cells. CD99 contains an extracellular O-glycosylated domain, a transmembrane domain and a short cytoplasmic domain. Human CD99 has multiple isoforms, one of which involves a deletion of amino acids 34-49 in the extracellular domain. Over the extracellular region used as immunogen, human CD99 is 42% and 51% identical to the mouse and canine protein sequences, respectively. Applications: Suitable for use in Immunocytochemistry, Flow Cytometry, ELISA and Western Blot. Other applications not tested. Recommended Dilutions: Flow Cytometry: 25ug/ml; 10ul will label 1-2.5 x 10e5 cells in a total reaction volume of 200ul. ELISA (Direct): 0.5-1ug/ml. The detection limit for rhCD99 is ~0.2ng/well. Western Blot: 0.1-0.2ug/ml. The detection limit for rhCD99 is ~0.5ng/lane under non-reducing and reducing conditions. Immunocytochemistry: 5-15ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice