C2443-81E
CD98 (4F2 Cell-surface Antigen Heavy Chain, 4F2 Heavy Chain Antigen, 4F2HC, 4T2HC, CD98HC, Lymphocyte Activation Antigen 4F2 Large Subunit, MDU1, NACAE, Solute Carrier Family 3 (Activators of Dibasic and Neutral Amino Acid Transport) Member 2, SLC3A2)
Size
£557.00
- SKU:
- C2443-81E
- Application:
- Flow Cytometry
- Purity:
- Purified by Protein A affinity chromatography from tissue culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Molt 13 T cell line.
- Clone:
- 7H227
- Format:
- Supplied as a liquid in 50mM sodium phosphate, pH 7.5, 100mM potassium chloride, 150mM sodium chloride. No preservative added.
- Extra Details:
- Human CD98 is expressed on monocytes and activated cells. All cultured human cell lines tested express CD98. CD98 is thought to be an activation antigen. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 5ug/ml (80ul) Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. Five x 10e5 cultured Raji human tumor cells were washed and incubated 45 minutes on ice with 80ul of C2443-81E at 5ug/ml. 2. Cells were washed twice and incubated with IgG (FITC) goat anti-mouse, after which they were washed three times, fixed and analyzed by FACS. 3. Cells stained positive with a mean shift of 1.57 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice