C2443-81A
CD98
Size
£557.00
- SKU:
- C2443-81A
- Application:
- Flow Cytometry
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Molt 13 T cell line.
- Clone:
- 7H227
- Format:
- Supplied as a liquid in PBS, pH 7.5, 0.5mg/ml Gentamicin Sulfate.
- Extra Details:
- Human CD98 is expressed on monocytes and activated cells. All cultured human cell lines tested express CD98. CD98 is thought to be an activation antigen. Applications: Suitable for use in Flow Cytometry and Functional Assay. Other applications not tested. Recommended Dilutions: Flow Cytometry: 1ug/ml. 80ul labels 5x10e5 Jurkat cells. Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. Five x 10e5 cultured Jurkat human tumor cells were washed and incubated 45 minutes on ice with 80ul of C2443-81A at 1ug/ml. 2. Cells were washed twice and incubated with a goat x mouse IgG (FITC) (I1904-06F). 3. Cells were washed three times, fixed and analyzed by FACS. 4. Cells stained positive with a mean shift of 1.82 log10 fluorescent units when compared to a Mouse IgG1 negative control (I1904-78R) at a similar concentration. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice