C2443-62F
CD97 (Leukocyte Antigen CD97, Seven Transmembrane Heterodimeric Receptor Associated with Inflammation, TM7LN1) (APC)
Size
£709.00
- SKU:
- C2443-62F
- Application:
- Flow Cytometry
- Purity:
- Purified by Protein G affinity chromatography from ascites.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- PHA-activated peripheral blood cells
- Clone:
- MEM-180
- Format:
- Supplied as a lyophilized powder in PBS, pH 7.4, 5% sucrose, 1% BSA, 0.09% sodium azide. Reconstitute with 1ml sterile ddH2O. Labeled with Allophycocyanin (APC).
- Extra Details:
- CD97 gene is a member of the EGF-TM7 family of class II seven-span transmembrane (7-TM) molecules, likely encoded by a gene cluster on the short arm of chromosome 19. The encoded product is a glycoprotein that is present on the surface of most activated leukocytes and spans the membrane seven times, which is a defining feature of G protein-coupled receptors. The protein has an extended extracellular region with several N-terminal epidermal growth factor (EGF)-like domains, which mediate binding to its cellular ligand, decay accelerating factor (DAF, CD55), a regulatory protein of the complement cascade. The presence of structural features characteristic of extracellular matrix proteins and transmembrane proteins suggests that this protein is a receptor involved in both cell adhesion and signaling processes early after leukocyte activation. Alternative splicing has been observed for this gene and three variants have been found. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: Neat-1:10. 10ul labels 10e6 cells or 100ul whole blood. Optimal diliution to be determined by researcher. Storage and Stability: Store product at 4°C before and after reconstitution. DO NOT FREEZE! Lyophilized and reconstituted products are stable for 12 months at 4°C after receipt. Reconstitute lyophilized powder with sterile ddH2O prior to use and store remainder as an undiluted liquid. Caution: PE conjugates are sensitive to light. For maximum recovery of product centrifuge the original vial prior to removing the cap.
- Shipping Conditions:
- Blue Ice