CD178 (sFas Ligand, Apoptosis Antigen Ligand, APT1LG1, APTL, CD95 Ligand, CD95L, CD95-L, FAS Antigen Ligand, Fas Ligand, FASL, FASLG, Tumor Necrosis Factor Ligand Superfamily Member 6, TNFSF6)

Size

£594.00

SKU:: C2442-32
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: Highly pure recombinant human FAS Ligand, expressed in CHO cells.
Format:: Supplied as a lyophilized powder from PBS, pH 7.4. Reconstitute with 100ul sterile dH2O.
Extra Details:: Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: ELISA: ≥0.5-2ug/ml by direct ELISA (using 100ul/well antibody solution). Allows the detection of 0.2-0.4ng/well of recombinant hFasL. Western Blot: 0.1-0.2ug/ml Detection limit for recombinant hFasL is 1.5-3ng/lane, under either reducing or non-reducing conditions. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.