C2442-09G
CD94 (Natural Killer Cells Antigen CD94, KP43, Killer Cell Lectin-like Receptor Subfamily D Member 1, NK Cell Receptor, KLRD1) (APC)
Size
£704.00
- SKU:
- C2442-09G
- Application:
- Flow Cytometry
- Purity:
- Purified by Protein G affinity chromatography from hybridoma culture supernatant.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- BaF3 mouse pro-B cell line transfected with human CD94 and NKG2A
- Clone:
- 12K45
- Format:
- Supplied as a liquid in saline, 0.5% BSA, 0.09% sodium azide. Labeled with Allophycocyanin (APC).
- Extra Details:
- CD94 is a 30kD type II transmembrane protein with an extracellular C-type lectin domain. It is expressed by natural killer cells and a subset of CD8+T cells, with cellular activation resulting in increased cell surface expression. Although CD94 can occur as a non-signaling homodimer, functional activity occurs when CD94 exists as a heterodimer with a NKG2 family member. The CD94/NKG2A heterodimer delivers an inhibitory signal to the expressing cell, whereas, the CD94/NKG2C heterodimer associates with the DAP12 adaptor protein and delivers an activating signal. Both heterodimeric complexes recognize HLA-E with an associated peptide derived from the signal peptide of other HLA proteins. Applications: Suitable for use in Flow Cytometry. Other applications have not been tested. Recommended Dilution: Flow Cytometry: 10ul labels 10e6 cells using human peripheral blood mononuclear cells (PBMCs) Optimal dilutions to be determined by the researcher. Excitation: 620-650nm Emission: 660-670nm Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
- Shipping Conditions:
- Blue Ice