C2442-07B-ML490
CD94 (CD94 Antigen, Killer Cell Lectin-like Receptor Subfamily D Member 1, KLRD1, KLRD1 Protein, Kp43, Natural Killer Cells Antigen CD94, NK Cell Receptor) (MaxLight 490)
Size
£837.00
- SKU:
- C2442-07B-ML490
- Application:
- Flow Cytometry
- Purity:
- Purified by Protein G affinity chromatography from tissue culture supernatant.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Rat
- Immunogen:
- CHO cells transfected with B6 allele of CD94.
- Clone:
- 18d3
- Format:
- Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™490.
- Extra Details:
- MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm); Emission (515nm); Extinction Coefficient 73,000. The murine CD94 molecule forms heterodimers with NKG2A, C or E subunits to form the CD94/NKG2 receptor. In mice the CD94/NKG2 receptor recognizes Qa-1b, a non-classical MHC class 1 molecule. CD94/NKG2 receptors may play an important role in self-tolerance of NK cells during fetal and neonatal life. N.B. Recent reports suggest that DBA/2J mice do not express cell surface CD94/NKG2A receptors (1). Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:50-1:200, Use 10ul to label 10e6 cells in 100ul. Optimal dilution determined by the researcher Hybridoma: P3X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice