C2442-02A
CD94 (KLRD1, Killer cell lectin-like receptor, subfamily D, member 1, Kp43) (Biotin)
Size
£639.00
- SKU:
- C2442-02A
- Application:
- Flow Cytometry
- Purity:
- Purified by Protein A affinity chromatography from tissue culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Cultured human NK cells.
- Clone:
- 7H191
- Format:
- Supplied as a liquid in PBS, 0.2% BSA, 0.04% sodium azide, 5% glycerol. Labeled with Biotin.
- Extra Details:
- Human CD94 expression is restricted to natural killer (NK) cells and a small subset of T lymphocytes. CD94 is a type II membrane glycoprotein of the c-type lectin superfamily and essentially lacks a cytoplasmic tail. Applications: Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry: Frozen sections Flow Cytometry: 5ug/ml (80ul) Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. Ficoll prepared human peripheral white blood cells were stimulated by incubating 1 day at 2x10e6 cells/ml in RPMI 10% FBS media including 5ug/ml Phytohemagglutinin-P. 2. Five x 10e5 cells per tube were then washed and preincubated ~10 minutes with 20ul human IgG at 250ug/ml (to block non-specific binding) after which they were incubated 45 minutes on ice with 80ul of C2442-02A at 5ug/ml. 3. Cells were washed twice and incubated with S7973-88P, secondary reagent Streptavidin/R-Phycoerythrin after which they were washed three times, fixed and analyzed by FACS. 4. A net 33% population of the cells stained positive with a mean shift of 1.57 log10 fluorescent units when compared to a Mouse IgG1/Biotin negative control at a similar concentration. 5. Binding was blocked when pre incubated 10 minutes with 20ul of C2442-02 at 0.5mg/ml. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice