C2441-91-ML650

CD91a (a2-Macroglobulin Receptor, a2MR) (MaxLight 650)

Size

£953.00

SKU:: C2441-91-ML650
Purity:: Purified by Protein A affinity chromatography from ascites
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Purified human CD91 alpha chain protein.
Clone:: 4H306
Format:: Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with MaxLight™650.
Extra Details:: MaxLight™ 650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000. CD91, also known as a2-macroglobulin receptor (a2MR1. Moestrup SK, et al. Cell Tissue Res 269:374-382, 1992. 2. Moestrup SK and Hokland P. Leuk Res 16(3):227-234, 1992. 3. Strickland DK, et al. J Biol Chem 265:17401-17404, 1990.), apolipoprotein E receptor (ApoER) and low density lipoprotein receptor-related protein (LRP), is a large two-chain membrane protein that binds the proteinase-activated form of the plasma membrane protein a2M.1 a2M is a proteinase-inhibitor containing a region that is a substrate for a wide range of proteinases including trypsin, chymotrypsin, plasmin, neutrophil elastase and fibroblast collagenase.1 Binding of a2M:proteinase complexes to a2MR is followed by endocytosis and degradation of the ligand in lysosomes. CD91 consists of two non-covalently associated polypeptides of ~420 to ~85kD, and has been shown to be present in a restricted spectrum of cell types, including neurons and astrocytes in the central nervous system, epithelial cells in the gastrointestinal tract, smooth muscle cells, fibroblasts, Leydig cells in testis, granulose cells in ovary, and dendritic interstitial cells of kidney.1 The high abundance of CD91 in certain cells types of most organs suggest two different mechanisms of CD91 -mediated ligand clearance.1 CD91 is expressed on malignant cells from patients with acute and chronic myelomonocytic leukemia, while no significant expression was found on malignant cells from acute and chronic lymphatic leukemia, lymphomas, plasma cell leukemias or hairy cell leukemia. CD91 was also shown to have a close correlation with CD14 surface expression. Applications: Suitable for use in Immunofluorescence, Flow Cytometry, Western Blot, Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunofluorescence: 10ug/ml Flow Cytometry: 10ug/ml Western Blot: 1ug/ml Immunohistochemistry (frozen): 10ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.

Shipping Conditions:: Blue Ice