C2441-90

CD91 (LRP, alpha2MR, Low Density Lipoprotein Receptor-related Protein)

Size

£754.00

SKU:: C2441-90
Purity:: Purified by Protein A affinity chromatography from tissue culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Purified alpha 2 macroglobulin receptor
Clone:: 3H1760 (Alpha 2MR alpha-2)
Format:: Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Extra Details:: CD91, a type I membrane protein which is also known as the alpha 2 macroglobulin receptor (alpha 2 MR). CD91, also known as Prolow-density lipoprotein receptor-related protein 1, Alpha-2-macroglobulin receptor or apolipoprotein E receptor. CD91 is a 4525aa protein post translationally cleaved into 3 subunits, a 85kD type I transmembrane carboxyl chain (LRP85) non-covalently bound to a 515kD extracellular N-terminal subunit (LRP515)containing multiple EGF-like and LDL-receptor Class A and Class B domains. Additionally, there is an intracellular domain (LRPICD) which can be cleaved from the transmambrane domain by gamma secretase (May et al. 2004). Clone A2Mr alpha-2 detects an epitope within the LRP515 chain. CD91 is a multifunctional protein involved in processes inluding the phagocytosis and endocytosis of apoptotic cells (Nilsson et al. 2012), clearance of activated serum alpha-2-macroglobulin (Kristensen et al. 1990), modulation of the inflammatory response (Staudt et al. 2013) and acts as a receptor for Pseudomonas aeruginosa exotoxin A (Kounnas et al. 1992). Applications: Suitable for use in Flow Cytometry, Immunohistochemistry, ELISA, Immunoprecipitation, Immunofluorescence and Western Blot. Other applications have not been tested. Recommended Dilutions: Flow Cytometry: 1:50-1:100. Use 10ul to label 10e6 cells in 100ul. Immunohistochemistry: Frozen sections Western Blot: Non-reducing conditions ELISA: Direct Optimal working dilutions to be determined by researcher. Recommended Secondary Antibodies: I1904-65W: IgG, F(ab’)2 (HRP) Pab RbxMo I1904-14C: IgG, Fc (HRP) Pab Gt xMo Recommended Negative Controls: I1904-78R: IgG1 Murine Negative Control Recommended Positive Control Tissue for IHC: Liver Hybridoma: NS1 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice