C2441-90-ML650
CD91 (LRP, alpha2MR, Low Density Lipoprotein Receptor-related Protein) (MaxLight 650)
Size
£869.00
- SKU:
- C2441-90-ML650
- Purity:
- Purified by Protein A affinity chromatography from tissue culture supernatant.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Purified alpha 2 macroglobulin receptor
- Clone:
- 3H1760 (Alpha 2MR alpha-2)
- Format:
- Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™650.
- Extra Details:
- MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000. CD91, a type I membrane protein which is also known as the alpha 2 macroglobulin receptor (alpha 2 MR). CD91, also known as Prolow-density lipoprotein receptor-related protein 1, Alpha-2-macroglobulin receptor or apolipoprotein E receptor. CD91 is a 4525aa protein post translationally cleaved into 3 subunits, a 85kD type I transmembrane carboxyl chain (LRP85) non-covalently bound to a 515kD extracellular N-terminal subunit (LRP515)containing multiple EGF-like and LDL-receptor Class A and Class B domains. Additionally, there is an intracellular domain (LRPICD) which can be cleaved from the transmambrane domain by gamma secretase (May et al. 2004). Clone A2Mr alpha-2 detects an epitope within the LRP515 chain. CD91 is a multifunctional protein involved in processes inluding the phagocytosis and endocytosis of apoptotic cells (Nilsson et al. 2012), clearance of activated serum alpha-2-macroglobulin (Kristensen et al. 1990), modulation of the inflammatory response (Staudt et al. 2013) and acts as a receptor for Pseudomonas aeruginosa exotoxin A (Kounnas et al. 1992). Applications: Suitable for use in Flow Cytometry, Immunohistochemistry, FLISA, Immunoprecipitation, Western Blot and Immunofluorescence. Other applications have not been tested. Recommended Dilutions: Immunohistochemistry: Frozen sections Western Blot: Non-reducing conditions Optimal working dilutions to be determined by researcher. Recommended Negative Controls: I1904-78R: IgG1 Murine Negative Control Recommended Positive Control Tissue for IHC: Liver Hybridoma: NS1 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice